Quantificação de glicose salivar por FT-IR e sua associação com marcadores glico-lipêmicos em trabalhadores rurais e da agroindústria.

Abstract

Introduction: In the monitoring of chronic degenerative diseases, the control of the glyco-lipemic profiles including glucose, total cholesterol, HDL, LDL and triglycerides, has been highlighted. These are contemplated with a new technology through FT-IR, focused on its diagnosis and clinical control. Objective: The objectives of this study were to develop and validate methods for salivary glucose quantification by FT-IR with reference to UV / Visible molecular absorption spectroscopy and to evaluate the association between salivary FT-IR spectra and respective profile values glycemic and salivary. Methodology: This is a cross-sectional study with 104 individuals of both sexes, aged over 18 years of the municipalities of Encruzilhada do Sul, Rio Pardo, Candelária, Passo do Sobrado and Vale Verde. Blood and salivary glucose measurements were performed by means of an analytical study, with biochemical and spectroscopic analyzes in the infrared. Summary of article 1: Salivary glucose was not shown to be a suitable biomarker to evaluate the glyco-lipemic profile in rural workers. Through the FT-IR infrared spectroscopy aided by chemometric method, it was possible to correlate salivary glucose with the glyco-lipemic parameters. Summary of the article 2: In order to complement and streamline the conventional clinical analyzes previously performed by enzymatic tests, the possibility arises through FT-IR based on a chemometric method, quantifying the salivary spectrum the glyco-lipemic parameters of fast, non-invasive and reagent free. Conclusion: Based on the findings in terms of cost and speed of results, FT-IR is an effective method for the quantification of salivary glucose by associating it with the glyco-lipemic profiles for rural workers who are part of this research; was an innovative and promising tool to associate the salivary spectrum in the correlation of the blood parameters of the subjects of this study.