Avaliação da atividade antifúngica in vitro de um bioproduto contendo óleo essencial de bergamota (Citrus reticulata b.) no tratamento de onicomicoses.

Abstract

The vegetable species Citrus reticulata Blanco better known as bergamot is a citrus fruit that has antifungal action in the essential oil and this action is associated with the main components such as monoterpenes. Onychomycosis is a fungal nail infection that accounts for approximately 50% of all nail disorders. The conventional treatment of topical and oral antifungals is often prolonged and ineffective for treating this infection. In this sense, this work aims to evaluate the antifungal activity of a bioproduct developed from the essential oil of Citrus reticulata and natural assets to be used in the treatment of onychomycosis. For this purpose, an evaluation of the antifungal activity of the bioproduct containing essential oil of C. reticulata against the dermatophyte fungi Microsporum canis and Trichophyton sp., and the yeasts, Candida glabrata and Candida tropicalis was carried out. The oil in the tested concentrations of 5, 10, 20 µL.mL-1 caused inhibition of 9.05%, 7.78% and 10.59% of mycelial growth, respectively for Microsporum canis and for the 2 clinical isolates of Trichophyton sp . 8.24% and 9.50% (5 µL.mL-1 ), 7.88% and 9.63% (10 µL.mL-1 ) and 2.25% (20 µL.mL-1 ). In the tested concentrations they didn’t show antifungal effect against Candida yeasts in the Minimum Inhibitory Concentration (MIC), however in the disc diffusion analysis it showed better inhibition halos in the concentrations of 20 µL.mL-1 , 12.31 (C. glabrata) and 8.28 (C. tropicalis). The results demonstrate that the bioproduct containing essential oil of bergamot has antifungal action against the fungi that cause onychomycosis Microsporum canis and Trichophyton sp. and the species of Candida tropicalis and Candida glabrata studied.