Potencial antimicrobiano de nanopartículas de prata produzidas por síntese verde.

Abstract

The World Health Organization (WHO) claims bacterial resistent is one of the main public health threats in the world in the 21st century. This happens due to the greater difficulty in the effective treatment of pathologies caused by bacteria and fungi of clinical origin. As a resultthere is an increase in public health expenses due to the extension of treatments and hospitalizations. The use of nanoparticles has gained prominence as an alternative to conventional treatments. Among the metallic nanoparticles used, silver nanoparticles (AgNPs) formed from green synthesis stand out. AgNPs are currently widely researched and considered as promising for the control of microbial multiresistance, since they can be transformed into nanoantibiotics and enhancers for those that already exist. When used in products for health, industry and the environment. Different biological organisms are capable of synthesizing nanoparticles using a safer, more efficient and ecologically correct method, among them, plants stand out, which produce extracts that act as metal ion reducers, transforming them into nanoparticles. The aim of this study was to evaluate the possible antimicrobial activity of silver nanoparticles synthesized from different plant extracts, honey and glycerol against bacterial isolates Pseudomonas aeruginosa, Staphylococcus aureus, Proteus mirabilis, Salmonella sp., Escherichia coli and Candida albicans yeast. To evaluate the antimicrobial activity of different nanoparticles, the well diffusion method was used. The minimum inhibitory concentration (MIC) was determined for the nanoparticles that showed antimicrobial activity. As a result, it was observed that of the eight silver nanoparticles tested, six showed antimicrobial activity against the tested microorganisms, with greater resistance to AgNPs by Gram-negative bacteria being observed. The MIC values observed show mostly inhibition at 32 ug/ml. Only the Gram negative bacteria Pseudomonas aeruginosa did not suffer the inhibitory action of any nanoparticles. The radish AgNP pH 11.4 (3) had the largest action spectrum. The silver nanoparticle synthesized with radish (pH 6.8) was the antimicrobial whose lowest concentration was able to prevent the visible growth of the fungus Candida albicans. It was concluded that AgNPs synthesized from natural products, mostly, inhibited, to some degree, the growth of the clinical isolates tested, showing this nanotechnology can be a promising alternative for the development and assistance of new antimicrobial products.